Epidemiological and mutational analysis of SARS-CoV-2


RECOVER-COVID19


RicErCa e sviluppO VERsus COVID19 in Campania

Source of the data

The lists of the AA mutations present in SARS-CoV-2 variants detected using the sequence of the Wuhan genome (GISAID accession ID: EPI_ISL_402124) as reference were retrieved from the Global Initiative for Sharing All Influenza Data (GISAID) database (https://www.gisaid.org/) at different time points.

Classification of the mutations

The AA substitutions were classified according to two criteria:

  • We considered the Point Accepted Mutation (PAM) score, i.e. the likelihood that an AA substitution is accepted by the natural selection.
  • We considered if the AA substitution required one or more than one base change in the genetic code to happen.

Differences in hydrophobicity (ΔHydrophobicity) between the mutated and the original residue was calculated based on the consensus hydrophobic scale developed by Eisenberg.